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>>>>Checklist<<<<
-Ez-Cloner
-T5 Fluorescent Lighting
-Permaclone collars or brand new neoprene collars (only permaclone should be reused)
-RO Water
-Gloves
-Sterile brush and scissors (I use a tooth brush)
-Bleach
-UC Roots (STERILIZING AGENT)
-Clonex cloning solution (NUTRIENTS)
-Hormex liquid (ROOTING HORMONE)
-PH equipment
>Initial Cleaning Step: (only if you've had cloner issues)<
- If you've experienced brown roots or stem/Pythium - using gloves, disassemble the cloner + pump entirely. Use brush to scrub each piece with a mixture of hot Water and ~5ml/gal bleach. Reassemble. This should only have to be done once in a blue moon.
>Standard Cleaning Step (repeat every time before taking cuttings)<
- Rinse Permaclone collars in hot water to remove any debris. Place rinsed collars back in cloner
- Fill cloner with mixture of hot water and 5ml/gal bleach. Run for 8 to 12 hours. Drain
- Fill with tap water and rinse for 2 minutes. Drain
- Remove Permaclone collars and sterilize using any of the Permaclone methods. (microwave/pressure cooker/etc.)
- Place sterilized collars back in cloner with gloves. Cloner is ready for use now.
>STEP 1<
- Fill with RO water from sterile reservoir
- Add 2-3 mL/gal Hormex
- PH to 6.0 using a clean calibrated PH probe. (I sterilize with rubbing alcohol and Q-tips but bleach works too)
- Turn cloner on
- Add 10 mL/gal UC Roots
- Let run for 15 minutes
>STEP 2<
- Wearing gloves, Take big clones with roughly 6 to 8 nodes on top. More nodes untrimmed = more natural auxins produced. Make the initial cut ~1/2" below a node
- Clean up lower stem roughly 2-3"
- No need to trim leaves/scrape stem/split stem in half
- Place all cuts in cups with mixture of RO water and 20mL/gal UC roots. This helps prevent embolism and is handy for transporting from room to room. I make ~1 gallon and label solo cups with strain names.
>STEP 3<
- Before placing in cloner, make a second final cut on each clone directly at the base of the node that is 1/2" above your initial cut. There is a plug of carbohydrates found at each node and it will aid in rooting. The secondary cut also helps prevent embolisms.
- Place clones into cloner with roughly 2-3 inches of stem to go below the collars.
>STEP 4<
- Add another 10 mL/gal Hormex and run for 15 minutes
- Add 20 mL/gal Clonex solution
>STEP 5<
- Add 2mL/gal UC roots every other day until final transplant
>STEP 6<
- On day 7 the stems should have a spongey white look from the Hormex (pic attached), Drain system and fill with fresh RO water from sterile reservoir
- PH to 6.0 using clean PH probe
- Turn cloner on
- Add 20 mL/gal UC roots, run for 15 mins
- Add 30-40ml/gal Clonex solution. Really big clones love the 40 mL/gal but if taking regular sized clones use 30mL/gal
>>>>ENVIRONMENT<<<<
- T5 Fluorescent lighting roughly 18" from canopy
- 78-80 deg. F
- ~65% Relative hum.
- lights and pump on 24/7